High-resolution, high-throughput analysis of <i>Drosophila</i> geotactic behavior.

ABSTRACT: Drosophila innate response to gravity, geotaxis, has been previously used to assess the impact of aging and disease on motor performance. Despite its rich history, fly geotaxis continues to be largely measured manually and assessed through simplistic metrics. The manual nature of this assay introduces substantial experimental variability while simplistic metrics provide limited analytic insights into the behavior. To address these shortcomings, we have constructed a fully automated, programable apparatus, and developed a multi-object tracking software capable of following sub-second movements of individual flies, thus allowing reproducible, detailed, and quantitative analysis of geotactic behavior. The apparatus triggers and monitors geotaxis of 10 fly cohorts simultaneously, with e

SUBMITTER: Canic T 

PROVIDER: S-EPMC11185704 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature