Genome-wide analysis of Smad and Schnurri transcription factors in <i>C. elegans</i> demonstrates widespread interaction and a function in collagen secretion.

ABSTRACT: Smads and their transcription factor partners mediate the transcriptional responses of target cells to secreted ligands of the Transforming Growth Factor-β (TGF-β) family, including those of the conserved bone morphogenetic protein (BMP) family, yet only a small number of direct target genes have been well characterized. In C. elegans, the BMP2/4 ortholog DBL-1 regulates multiple biological functions, including body size, via a canonical receptor-Smad signaling cascade. Here, we identify functional binding sites for SMA-3/Smad and its transcriptional partner SMA-9/Schnurri based on ChIP-seq peaks (identified by modEncode) and expression differences of nearby genes identified from RNA-seq analysis of corresponding mutants. We found that SMA-3 and SMA-9 have both overlapping and uniqu

SUBMITTER: Vora M 

PROVIDER: S-EPMC11185707 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature