Tracking protein-protein interactions by NMR: conformational selection in human steroidogenic cytochrome P450 CYP17A1 induced by cytochrome <i>b</i>₅.

ABSTRACT: The human steroidogenic cytochrome P450 CYP17A1 catalyzes two types of reactions in the biosynthetic pathway leading from pregnenolone to testosterone and several other steroid hormones. The first is the hydroxylation of pregnenolone or progesterone to the corresponding 17α-hydroxy steroid, followed by a lyase reaction that converts these 17α-hydroxy intermediates to the androgens dehydroepiandrosterone and androstenedione, respectively. cytochrome b₅ (cytb₅) is known to act as both an effector and electron donor for the lyase oxidations, markedly stimulating the rate of the lyase reaction in its presence relative to the rate in its absence. Extensive sequential backbone ¹H,¹⁵N and ¹³C nuclear magnetic resonance assignmen

SUBMITTER: Richard AM 

PROVIDER: S-EPMC11186455 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature