Cell cycle-dependent centrosome clustering precedes proplatelet formation.

ABSTRACT: Platelet-producing megakaryocytes (MKs) primarily reside in the bone marrow, where they duplicate their DNA content with each cell cycle resulting in polyploid cells with an intricate demarcation membrane system. While key elements of the cytoskeletal reorganizations during proplatelet formation have been identified, what initiates the release of platelets into vessel sinusoids remains largely elusive. Using a cell cycle indicator, we observed a unique phenomenon, during which amplified centrosomes in MKs underwent clustering following mitosis, closely followed by proplatelet formation, which exclusively occurred in G₁ of interphase. Forced cell cycle arrest in G₁ increased proplatelet formation not only in vitro but also in vivo following short-term starvation of mic

SUBMITTER: Becker IC 

PROVIDER: S-EPMC11186502 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature