Two transcriptionally and functionally distinct waves of neutrophils during mouse acute liver injury.

ABSTRACT:

Background

Neutrophils are key mediators of inflammation during acute liver injury (ALI). Emerging evidence suggests that they also contribute to injury resolution and tissue repair. However, the different neutrophil subsets involved in these processes and their kinetics are undefined. Herein, we characterized neutrophil kinetics and heterogeneity during ALI.

Methods

We used the carbon tetrachloride model of ALI and employed flow cytometry, tissue imaging, and quantitative RT-PCR to characterize intrahepatic neutrophils during the necroinflammatory early and late repair phases of the wound healing response to ALI. We FACS sorted intrahepatic neutrophils at key time points and examined their transcriptional profiles using RNA-sequencing. Finally, we evaluated neutrophil prote

SUBMITTER: Maali Y 

PROVIDER: S-EPMC11186811 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature