Exploring the Cocktail Factor Approach to Generate Salivary Gland Progenitors through Co-Culture Techniques.

ABSTRACT:

Background

The derivation of salivary gland (SG) progenitors from pluripotent stem cells (PSCs) presents significant potential for developmental biology and regenerative medicine. However, the existing protocols for inducing SG include limited factors, making it challenging to mimic the in vivo microenvironment of embryonic SGs.

Methods

We reported a cocktail factor approach to promote the differentiation of mouse embryonic stem cell (mESC)-derived oral epithelium (OE) into SG progenitors through a three-dimensional co-culture method. Upon confirming that the embryonic SG can promote the differentiation of mESC-derived OE, we performed RNA sequence analysis to identify factors involved in the differentiation of SG progenitors.

Results

Our findings highlight several ef

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC11187051 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature