Small molecule in situ resin capture provides a compound first approach to natural product discovery.

ABSTRACT: Culture-based microbial natural product discovery strategies fail to realize the extraordinary biosynthetic potential detected across earth's microbiomes. Here we introduce Small Molecule In situ Resin Capture (SMIRC), a culture-independent method to obtain natural products directly from the environments in which they are produced. We use SMIRC to capture numerous compounds including two new carbon skeletons that were characterized using NMR and contain structural features that are, to the best of our knowledge, unprecedented among natural products. Applications across diverse marine habitats reveal biome-specific metabolomic signatures and levels of chemical diversity in concordance with sequence-based predictions. Expanded deployments, in situ cultivation, and metagenomics facilitate com

SUBMITTER: Bogdanov A 

PROVIDER: S-EPMC11187115 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature