Physiological platelet aggregation assay to mitigate drug-induced thrombocytopenia using a microphysiological system.

ABSTRACT: Developing a reliable method to predict thrombocytopenia is imperative in drug discovery. Here, we establish an assay using a microphysiological system (MPS) to recapitulate the in-vivo mechanisms of platelet aggregation and adhesion. This assay highlights the role of shear stress on platelet aggregation and their interactions with vascular endothelial cells. Platelet aggregation induced by soluble collagen was detected under agitated, but not static, conditions using a plate shaker and gravity-driven flow using MPS. Notably, aggregates adhered on vascular endothelial cells under gravity-driven flow in the MPS, and this incident increased in a concentration-dependent manner. Upon comparing the soluble collagen-induced aggregation activity in platelet-rich plasma (PRP) and whole blood, rema

SUBMITTER: Harada K 

PROVIDER: S-EPMC11187140 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature