DNA replication in early mammalian embryos is patterned, predisposing lamina-associated regions to fragility.

ABSTRACT: DNA replication in differentiated cells follows a defined program, but when and how it is established during mammalian development is not known. Here we show using single-cell sequencing, that late replicating regions are established in association with the B compartment and the nuclear lamina from the first cell cycle after fertilization on both maternal and paternal genomes. Late replicating regions contain a relative paucity of active origins and few but long genes and low G/C content. In both bovine and mouse embryos, replication timing patterns are established prior to embryonic genome activation. Chromosome breaks, which form spontaneously in bovine embryos at sites concordant with human embryos, preferentially locate to late replicating regions. In mice, late replicating regions sho

SUBMITTER: Xu S 

PROVIDER: S-EPMC11187207 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature