Absence of a pancreatic microbiome in intraductal papillary mucinous neoplasm.

ABSTRACT:

Objective

This study aims to validate the existence of a microbiome within intraductal papillary mucinous neoplasm (IPMN) that can be differentiated from the taxonomically diverse DNA background of next-generation sequencing procedures.

Design

We generated 16S rRNA amplicon sequencing data to analyse 338 cyst fluid samples from 190 patients and 19 negative controls, the latter collected directly from sterile syringes in the operating room. A subset of samples (n=20) and blanks (n=5) were spiked with known concentrations of bacterial cells alien to the human microbiome to infer absolute abundances of microbial traces. All cyst fluid samples were obtained intraoperatively and included IPMNs with various degrees of dysplasia as well as other cystic neoplasms. Follow-up culturin

SUBMITTER: Pust MM 

PROVIDER: S-EPMC11187374 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature