Identification of Various Recombinants in a Patient Coinfected With the Different SARS-CoV-2 Variants.

ABSTRACT:

Background

Viral recombination that occurs by exchanging genetic materials between two viral genomes coinfecting the same host cells is associated with the emergence of new viruses with different virulence. Herein, we detected a patient coinfected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Delta and Omicron variants and identified various recombinants in the SARS-CoV-2 full-length spike gene using long-read and Sanger sequencing.

Methods

Samples from five patients in Japan with household transmission of coronavirus disease 2019 (COVID-19) were analyzed using molecular assays for detection and identification of SARS-CoV-2. Whole-genome sequencing was conducted using multiplex PCR with short-read sequencing.

Results

Among the five SARS-CoV-2-posit

SUBMITTER: Sayama Y 

PROVIDER: S-EPMC11187932 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature