Neto proteins differentially modulate the gating properties of Drosophila NMJ glutamate receptors.

ABSTRACT: The formation of functional synapses requires co-assembly of ion channels with their accessory proteins which controls where, when, and how neurotransmitter receptors function. The auxiliary protein Neto modulates the function of kainate-type glutamate receptors in vertebrates as well as at the Drosophila neuromuscular junction (NMJ), a glutamatergic synapse widely used for genetic studies on synapse development. We previously reported that Neto is essential for the synaptic recruitment and function of glutamate receptors. Here, using outside-out patch-clamp recordings and fast ligand application, we examine for the first time the biophysical properties of recombinant Drosophila NMJ receptors expressed in HEK293T cells and compare them with native receptor complexes of geneti

SUBMITTER: Han TH 

PROVIDER: S-EPMC11188076 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature