High resolution maps of chromatin reorganization through mouse meiosis reveal novel features of the 3D meiotic structure.

ABSTRACT: When germ cells transition from the mitotic cycle into meiotic prophase I (MPI), chromosomes condense into an array of chromatin loops that are required to promote homolog pairing and genetic recombination. To identify the changes in chromosomal conformation, we isolated nuclei on a trajectory from spermatogonia to the end of MPI. At each stage along this trajectory, we built genomic interaction maps with the highest temporal and spatial resolution to date. The changes in chromatin folding coincided with a concurrent decline in mitotic cohesion and a rise in meiotic cohesin complexes. We found that the stereotypical large-scale A and B compartmentalization was lost during meiotic prophase I alongside the loss of topological associating domains (TADs). Still, local subcompartments were dete

SUBMITTER: Cheng G 

PROVIDER: S-EPMC11188084 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature