Crosstalk of growth factor receptors at plasma membrane clathrin-coated sites.

ABSTRACT: Cellular communication is regulated at the plasma membrane by the interactions of receptor, adhesion, signaling, exocytic, and endocytic proteins. Yet, the composition and control of these nanoscale complexes in response to external cues remain unclear. Here, we use high-resolution and high-throughput fluorescence imaging to map the localization of growth factor receptors and related proteins at single clathrin-coated structures across the plasma membrane of human squamous HSC3 cells. We find distinct protein signatures between control cells and cells stimulated with ligands. Clathrin sites at the plasma membrane are preloaded with some receptors but not others. Stimulation with epidermal growth factor induces a capture and concentration of epidermal growth factor-, fibroblast growth facto

SUBMITTER: Alfonzo-Mendez MA 

PROVIDER: S-EPMC11188102 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature