Genome-wide screen of genetic determinants that govern Escherichia coli growth and persistence in lake water.

ABSTRACT: Although enteric bacteria normally reside within the animal intestine, the ability to persist extraintestinally is an essential part of their overall lifestyle, and it might contribute to transmission between hosts. Despite this potential importance, few genetic determinants of extraintestinal growth and survival have been identified, even for the best-studied model, Escherichia coli. In this work, we thus used a genome-wide library of barcoded transposon insertions to systematically identify functional clusters of genes that are crucial for E. coli fitness in lake water. Our results revealed that inactivation of pathways involved in maintaining outer membrane integrity, nucleotide biosynthesis, and chemotaxis negatively affected E. coli growth or survival in this extraintestinal environme

SUBMITTER: Teteneva N 

PROVIDER: S-EPMC11188689 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature