Detection of phosphorylation post-translational modifications along single peptides with nanopores.

ABSTRACT: Current methods to detect post-translational modifications of proteins, such as phosphate groups, cannot measure single molecules or differentiate between closely spaced phosphorylation sites. We detect post-translational modifications at the single-molecule level on immunopeptide sequences with cancer-associated phosphate variants by controllably drawing the peptide through the sensing region of a nanopore. We discriminate peptide sequences with one or two closely spaced phosphates with 95% accuracy for individual reads of single molecules.

SUBMITTER: Nova IC 

PROVIDER: S-EPMC11189593 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature