MICU3 Regulates Mitochondrial Calcium and Cardiac Hypertrophy.

ABSTRACT:

Background

Calcium (Ca²⁺) uptake by mitochondria occurs via the mitochondrial Ca²⁺ uniporter. Mitochondrial Ca²⁺ uniporter exists as a complex, regulated by 3 MICU (mitochondrial Ca²⁺ uptake) proteins localized in the intermembrane space: MICU1, MICU2, and MICU3. Although MICU3 is present in the heart, its role is largely unknown.

Methods

We used CRISPR-Cas9 to generate a mouse with global deletion of MICU3 and an adeno-associated virus (AAV9) to overexpress MICU3 in wild-type mice. We examined the role of MICU3 in regulating mitochondrial calcium ([Ca²⁺]_m) in ex vivo hearts using an optical method following adrenergic stimulation in perfused hearts loaded with a Ca²⁺-sensitive fluorophore. Addition

SUBMITTER: Roman B 

PROVIDER: S-EPMC11189743 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature