Expanding the genetic toolbox for the obligate human pathogen Streptococcus pyogenes.

ABSTRACT: Genetic tools form the basis for the study of molecular mechanisms. Despite many recent advances in the field of genetic engineering in bacteria, genetic toolsets remain scarce for non-model organisms, such as the obligatory human pathogen Streptococcus pyogenes. To overcome this limitation and enable the straightforward investigation of gene functions in S. pyogenes, we have developed a comprehensive genetic toolset. By adapting and combining different tools previously applied in other Gram-positive bacteria, we have created new replicative and integrative plasmids for gene expression and genetic manipulation, constitutive and inducible promoters as well as fluorescence reporters for S. pyogenes. The new replicative plasmids feature low- and high-copy replicons combin

SUBMITTER: Lautenschlager N 

PROVIDER: S-EPMC11190166 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature