Rapid and sensitive detection of glucocorticoids using engineered magnetosomes functionalized protein A conjugated broad-spectrum monoclonal antibody.

ABSTRACT: Engineered bacterial magnetic nanoparticles (BMPs) fused with protein A (BMP-PA) can bind antibodies, creating immunomagnetic beads that offer an attractive tool for targets screening. In the study, BMP-PA-IgG was formed by attaching broad-spectrum monoclonal antibodies against glucocorticoids (GCs) to BMP-PA. Immunomagnetic assay was developed for analysis of GCs, using the BMP-PA-IgG and hydrocortisone-horseradish peroxidase. The developed assay exhibited broad specificity for GCs, including hydrocortisone (HCS), betamethasone (BMS), dexamethasone (DMS), prednisolone (PNS), beclomethasone (BCMS), cortisone (CS), 6-α-methylprednisone (6-α-MPNS), and fludrocortisone acetate (HFCS), with half inhibitory concentrations (IC₅₀) ranging from 0.88 to 6.57 ng/mL. The proposed assay sho

SUBMITTER: He J 

PROVIDER: S-EPMC11190481 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature