UBQLN1 links proteostasis and mitochondria function to telomere maintenance in human embryonic stem cells.

ABSTRACT:

Background

Telomeres consist of repetitive DNA sequences at the chromosome ends to protect chromosomal stability, and primarily maintained by telomerase or occasionally by alternative telomere lengthening of telomeres (ALT) through recombination-based mechanisms. Additional mechanisms that may regulate telomere maintenance remain to be explored. Simultaneous measurement of telomere length and transcriptome in the same human embryonic stem cell (hESC) revealed that mRNA expression levels of UBQLN1 exhibit linear relationship with telomere length.

Methods

In this study, we first generated UBQLN1-deficient hESCs and compared with the wild-type (WT) hESCs the telomere length and molecular change at RNA and protein level by RNA-seq and proteomics. Then we identified the potential

SUBMITTER: Zhao S 

PROVIDER: S-EPMC11191273 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature