Fractionation of Extracellular Polymeric Substances by Aqueous Three-Phase Partitioning Systems.

ABSTRACT: Extracellular polymeric substances (EPS) are natural polymers secreted by microorganisms and represent a key chemical for the development of a range of circular economy applications. The production of EPS comes with notable challenges such as downstream processing. In this work, a three-phase partitioning (TPP) system was investigated as a fractionation technique for the separation of polysaccharides and proteins, both present in the EPS culture broth. The effect of the type of phase-forming compounds (alcohol, polymer, or ionic liquid, in combination with salt) and its concentration were evaluated and compared to the results previously obtained with model systems. The recyclability of phase-forming compounds used to form the fractionation platform was assessed by ultrafiltration. The best

SUBMITTER: Antunes EC 

PROVIDER: S-EPMC11191973 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature