Engineered extracellular vesicles from human skin cells induce pro-β-cell conversions in pancreatic ductal cells.

ABSTRACT: Direct nuclear reprogramming has the potential to enable the development of β cell replacement therapies for diabetes that do not require the use of progenitor/stem cell populations. However, despite their promise, current approaches to β cell-directed reprogramming rely heavily on the use of viral vectors. Here we explored the use of extracellular vesicles (EVs) derived from human dermal fibroblasts (HDFs) as novel non-viral carriers of endocrine cell-patterning transcription factors, to transfect and transdifferentiate pancreatic ductal epithelial cells (PDCs) into hormone-expressing cells. Electrotransfection of HDFs with expression plasmids for Pdx1, Ngn3, and MafA (PNM) led to the release of EVs loaded with PNM at the gene, mRNA, and protein level. E

SUBMITTER: Ortega-Pineda L 

PROVIDER: S-EPMC11192446 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature