Multifunctional hydrogels with spatially controlled light activation with photocaged oligonucleotides.

ABSTRACT: Recreating tissue environments with precise control over mechanical, biochemical, and cellular organization is essential for next-generation tissue models for drug discovery, development studies, and the replication of disease environments. However, controlling these properties at cell-scale lengths remains challenging. Here, we report the development of printing approaches that leverage polyethylene glycol diacrylate (PEGDA) hydrogels containing photocaged oligonucleotides to spatially program material characteristics with non-destructive, non-ultraviolet light. We further integrate this system with a perfusion chamber to allow us to alter the composition of PEGDA hydrogels while retaining common light-activatable photocaged DNAs. We demonstrate that the hydrogels can capture DNA function

SUBMITTER: Mathis K 

PROVIDER: S-EPMC11192495 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature