SETDB1 deletion causes DNA demethylation and upregulation of multiple zinc-finger genes.

ABSTRACT:

Background

SETDB1 (SET domain bifurcated-1) is a histone H3-lysine 9 (H3K9)-specific methyltransferase that mediates heterochromatin formation and repression of target genes. Despite the assumed functional link between DNA methylation and SETDB1-mediated H3K9 trimethylations, several studies have shown that SETDB1 operates autonomously of DNA methylation in a region- and cell-specific manner. This study analyzes SETDB1-null HAP1 cells through a linked methylome and transcriptome analysis, intending to explore genes controlled by SETDB1-involved DNA methylation.

Methods and results

We investigated SETDB1-mediated regulation of DNA methylation and gene transcription in human HAP1 cells using reduced-representation bisulfite sequencing (RRBS) and RNA sequencing. While two-third

SUBMITTER: Kang YK 

PROVIDER: S-EPMC11192681 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature