A side-by-side comparison of different capacitation media in developing mouse sperm fertilizing ability.

ABSTRACT: To acquire the ability to fertilize the egg, mammalian spermatozoa must undergo a series of changes occurring within the highly synchronized and specialized environment of the female reproductive tract, collectively known as capacitation. In an attempt to replicate this process in vitro, various culture media for mouse sperm were formulated over the past decades, sharing a similar overall composition but differing mainly in ion concentrations and metabolic substrates. The widespread use of the different media to study the mechanisms of capacitation might hinder a comprehensive understanding of this process, as the medium could become a confounding variable in the analysis. In this context, the present side-by-side study compares the influence of four commonly used culture media (FD, HTF an

SUBMITTER: Gonzalez LN 

PROVIDER: S-EPMC11192932 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature