Lymphoblastoid cell lines do not recapitulate physiological circulating B cell subtypes.

ABSTRACT: Lymphoblastoid cell lines (LCLs) are immortalised peripheral B lymphocytes, transformed via infection with Epstein Barr virus (EBV). The use of LCLs to study B cell function remains controversial and core markers to define physiological B cell populations are not consistent between studies of physiological B cells and LCLs. A consensus on the nature of these commonly used cell lines has not been reached. Recently, a core set of markers to subtype peripheral B cells was proposed, addressing the lack of agreed markers for B cell characterisation. In this present study, the consensus panel was applied to describe the B cell subtypes in LCLs. We found that LCLs were generally not physiologically representative of B cells, with most cells harbouring marker combinations absent on peripheral B ce

SUBMITTER: MacKinnon C 

PROVIDER: S-EPMC11192983 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature