Comparison of Novel and Traditional Bleeding Techniques in Neonatal and Juvenile Mice.

ABSTRACT: Blood collection is frequently used for neonatal and juvenile mice in toxicology, developmental, and immunology studies and is often a terminal procedure. However, the use of nonterminal blood collection techniques, including the submandibular and the submental collection techniques described for adult mice, may offer opportunities to reduce animal numbers and refine current methods. The use of the submental technique has not been described for neonatal or juvenile mice. In this study, we compared the submental and submandibular blood collection techniques to determine their suitability for use in neonatal and juvenile mice. Male and female CD1 mice, ages 7, 14, 21, and 28 d, were randomized by sex into submental (n = 16), submandibular (n = 16), or control (n = 8) groups. Each mouse was w

SUBMITTER: Prentiss RL 

PROVIDER: S-EPMC11193428 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature