Strain dynamics of contaminating bacteria modulate the yield of ethanol biorefineries.
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ABSTRACT: Bioethanol is a sustainable energy alternative and can contribute to global greenhouse-gas emission reductions by over 60%. Its industrial production faces various bottlenecks, including sub-optimal efficiency resulting from bacteria. Broad-spectrum removal of these contaminants results in negligible gains, suggesting that the process is shaped by ecological interactions within the microbial community. Here, we survey the microbiome across all process steps at two biorefineries, over three timepoints in a production season. Leveraging shotgun metagenomics and cultivation-based approaches, we identify beneficial bacteria and find improved outcome when yeast-to-bacteria ratios increase during fermentation. We provide a microbial gene catalogue which reveals bacteria-specific pathways associa
SUBMITTER: de Oliveira Lino FS
PROVIDER: S-EPMC11193817 | biostudies-literature | 2024 Jun
REPOSITORIES: biostudies-literature
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