Regulation of gene editing using T-DNA concatenation.

ABSTRACT: Transformation via Agrobacterium tumefaciens is the predominant method used to introduce exogenous DNA into plant genomes^1,2. Transfer DNA (T-DNA) originating from Agrobacterium can be integrated as a single copy or in complex concatenated forms^3,4, but the mechanisms affecting final T-DNA structure remain unknown. Here we demonstrate that inclusion of retrotransposon (RT)-derived sequences in T-DNA can increase T-DNA copy number by more than 50-fold in Arabidopsis thaliana. These additional T-DNA copies are organized into large concatemers, an effect primarily induced by the long terminal repeats (LTRs) of RTs that can be replicated using non-LTR DNA repeats. We found that T-DNA concatenation is dependent on the activity of the DNA repair proteins MRE11, RAD17 and AT

SUBMITTER: Dickinson L 

PROVIDER: S-EPMC11193869 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature