Simultaneous profiling of chromatin accessibility and DNA methylation in complete plant genomes using long-read sequencing.

ABSTRACT: Epigenetic regulations, including chromatin accessibility, nucleosome positioning and DNA methylation intricately shape genome function. However, current chromatin profiling techniques relying on short-read sequencing technologies fail to characterise highly repetitive genomic regions and cannot detect multiple chromatin features simultaneously. Here, we performed Simultaneous Accessibility and DNA Methylation Sequencing (SAM-seq) of purified plant nuclei. Thanks to the use of long-read nanopore sequencing, SAM-seq enables high-resolution profiling of m6A-tagged chromatin accessibility together with endogenous cytosine methylation in plants. Analysis of naked genomic DNA revealed significant sequence preference biases of m6A-MTases, controllable through a normalisation step. By applying SA

SUBMITTER: Leduque B 

PROVIDER: S-EPMC11194078 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature