Extensive long-range polycomb interactions and weak compartmentalization are hallmarks of human neuronal 3D genome.

ABSTRACT: Chromatin architecture regulates gene expression and shapes cellular identity, particularly in neuronal cells. Specifically, polycomb group (PcG) proteins enable establishment and maintenance of neuronal cell type by reorganizing chromatin into repressive domains that limit the expression of fate-determining genes and sustain distinct gene expression patterns in neurons. Here, we map the 3D genome architecture in neuronal and non-neuronal cells isolated from the Wernicke's area of four human brains and comprehensively analyze neuron-specific aspects of chromatin organization. We find that genome segregation into active and inactive compartments is greatly reduced in neurons compared to other brain cells. Furthermore, neuronal Hi-C maps reveal strong long-range interactions, forming a speci

SUBMITTER: Pletenev IA 

PROVIDER: S-EPMC11194087 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature