Cell-type-resolved mosaicism reveals clonal dynamics of the human forebrain.

ABSTRACT: Debate remains around the anatomical origins of specific brain cell subtypes and lineage relationships within the human forebrain^1-7. Thus, direct observation in the mature human brain is critical for a complete understanding of its structural organization and cellular origins. Here we utilize brain mosaic variation within specific cell types as distinct indicators for clonal dynamics, denoted as cell-type-specific mosaic variant barcode analysis. From four hemispheres and two different human neurotypical donors, we identified 287 and 780 mosaic variants, respectively, that were used to deconvolve clonal dynamics. Clonal spread and allele fractions within the brain reveal that local hippocampal excitatory neurons are more lineage-restricted than resident neocortical excitatory n

SUBMITTER: Chung C 

PROVIDER: S-EPMC11194162 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature