An Extremely Sensitive Ultra-High Throughput Growth Selection Assay for the Identification of Amidase Activity.

ABSTRACT: In the last decades, biocatalysis has offered new perspectives for the synthesis of (chiral) amines, which are essential building blocks for pharmaceuticals, fine and bulk chemicals. In this regard, amidases have been employed due to their broad substrate scope and their independence from expensive cofactors. To expand the repertoire of amidases, tools for their rapid identification and characterization are greatly demanded. In this work an ultra-high throughput growth selection assay based on the production of the folate precursor p-aminobenzoic acid (PABA) is introduced to identify amidase activity. PABA-derived amides structurally mimic the broad class of commonly used chromogenic substrates derived from p-nitroaniline. This suggests that the assay should be broadly applicable for the i

SUBMITTER: Branson Y 

PROVIDER: S-EPMC11194204 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature