The surface modification of the silica-coated magnetic nanoparticles and their application in molecular diagnostics of virus infection.

ABSTRACT: The study presents a series of examples of magnetic nanoparticle systems designed for the diagnosis of viral diseases. In this interdisciplinary work, we describe one of the most comprehensive synthetic approaches for the preparation and functionalization of smart nanoparticle systems for rapid and effective RT-PCR diagnostics and isolation of viral RNA. Twelve different organic ligands and inorganic porous silica were used for surface functionalization of the Fe₃O₄ magnetic core to increase the number of active centres for efficient RNA binding from human swab samples. Different nanoparticle systems with common beads were characterized by HRTEM, SEM, FT-IR, XRD, XPS and magnetic measurements. We demonstrate the application of the fundamental models modified to fit th

SUBMITTER: Zelenakova A 

PROVIDER: S-EPMC11194262 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature