A highly reproducible and efficient method for retinal organoid differentiation from human pluripotent stem cells.

ABSTRACT: Human pluripotent stem cell (hPSC)-derived retinal organoids are three-dimensional cellular aggregates that differentiate and self-organize to closely mimic the spatial and temporal patterning of the developing human retina. Retinal organoid models serve as reliable tools for studying human retinogenesis, yet limitations in the efficiency and reproducibility of current retinal organoid differentiation protocols have reduced the use of these models for more high-throughput applications such as disease modeling and drug screening. To address these shortcomings, the current study aimed to standardize prior differentiation protocols to yield a highly reproducible and efficient method for generating retinal organoids. Results demonstrated that through regulation of organoid size and shape using

SUBMITTER: Harkin J 

PROVIDER: S-EPMC11194494 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature