A Lifeact-EGFP quail for studying actin dynamics in vivo.

ABSTRACT: Here, we report the generation of a transgenic Lifeact-EGFP quail line for the investigation of actin organization and dynamics during morphogenesis in vivo. This transgenic avian line allows for the high-resolution visualization of actin structures within the living embryo, from the subcellular filaments that guide cell shape to the supracellular assemblies that coordinate movements across tissues. The unique suitability of avian embryos to live imaging facilitates the investigation of previously intractable processes during embryogenesis. Using high-resolution live imaging approaches, we present the dynamic behaviors and morphologies of cellular protrusions in different tissue contexts. Furthermore, through the integration of live imaging with computational segmentation, we visualize cel

SUBMITTER: Alvarez YD 

PROVIDER: S-EPMC11194674 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature