Distinct subcellular localisation of intramyocellular lipids and reduced PKCε/PKCθ activity preserve muscle insulin sensitivity in exercise-trained mice.

ABSTRACT:

Aims/hypothesis

Athletes exhibit increased muscle insulin sensitivity, despite increased intramuscular triacylglycerol content. This phenomenon has been coined the 'athlete's paradox' and is poorly understood. Recent findings suggest that the subcellular distribution of sn-1,2-diacylglycerols (DAGs) in the plasma membrane leading to activation of novel protein kinase Cs (PKCs) is a crucial pathway to inducing insulin resistance. Here, we hypothesised that regular aerobic exercise would preserve muscle insulin sensitivity by preventing increases in plasma membrane sn-1,2-DAGs and activation of PKCε and PKCθ despite promoting increases in muscle triacylglycerol content.

Methods

C57BL/6J mice were allocated to three groups (regular chow feeding [RC]; high-fat diet feeding [HFD]

SUBMITTER: Gaspar RC 

PROVIDER: S-EPMC11194860 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature