Mg²⁺-dependent mechanism of environmental versatility in a multidrug efflux pump.

ABSTRACT: Tripartite resistance nodulation and cell division multidrug efflux pumps span the periplasm and are a major driver of multidrug resistance among Gram-negative bacteria. The periplasm provides a distinct environment between the inner and outer membranes of Gram-negative bacteria. Cations, such as Mg²⁺, become concentrated within the periplasm and, in contrast to the cytoplasm, its pH is sensitive to conditions outside the cell. Here, we reveal an interplay between Mg²⁺ and pH in modulating the dynamics of the periplasmic adaptor protein, AcrA, and its function within the prototypical AcrAB-TolC multidrug efflux pump from Escherichia coli. In the absence of Mg²⁺, AcrA becomes increasingly plastic within acidic conditions, but when Mg²⁺ is b

SUBMITTER: Lewis BR 

PROVIDER: S-EPMC11195059 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature