Targeted mutagenesis of specific genomic DNA sequences in animals for the <i>in vivo</i> generation of variant libraries.

ABSTRACT: Understanding how the number, placement and affinity of transcription factor binding sites dictates gene regulatory programs remains a major unsolved challenge in biology, particularly in the context of multicellular organisms. To uncover these rules, it is first necessary to find the binding sites within a regulatory region with high precision, and then to systematically modulate this binding site arrangement while simultaneously measuring the effect of this modulation on output gene expression. Massively parallel reporter assays (MPRAs), where the gene expression stemming from 10,000s of in vitro-generated regulatory sequences is measured, have made this feat possible in high-throughput in single cells in culture. However, because of lack of technologies to incorporate DNA libraries, MPR

SUBMITTER: Falo-Sanjuan J 

PROVIDER: S-EPMC11195090 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature