Spatial Single-cell Analysis Decodes Cortical Layer and Area Specification.

ABSTRACT: The human cerebral cortex, pivotal for advanced cognitive functions, is composed of six distinct layers and dozens of functionally specialized areas^1,2. The layers and areas are distinguished both molecularly, by diverse neuronal and glial cell subtypes, and structurally, through intricate spatial organization^3,4. While single-cell transcriptomics studies have advanced molecular characterization of human cortical development, a critical gap exists due to the loss of spatial context during cell dissociation^5,6,7,8. Here, we utilized multiplexed error-robust fluorescence in situ hybridization (MERFISH)⁹, augmented with deep-learning-based cell segmentation, to examine the molecular, cellular, and cytoarchitectural development of human fetal cortex

SUBMITTER: Qian X 

PROVIDER: S-EPMC11195106 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature