The impact of YabG mutations on C. difficile spore germination and processing of spore substrates.

ABSTRACT: YabG is a sporulation-specific protease that is conserved among sporulating bacteria. C. difficile YabG processes cortex destined proteins preproSleC into proSleC and CspBA to CspB and CspA. YabG also affects synthesis of spore coat/exosporium proteins CotA and CdeM. In prior work that identified CspA as the co-germinant receptor, mutations in yabG were found which altered the co-germinants required to initiate spore germination. To understand how these mutations in the yabG locus contribute to C. difficile spore germination, we introduced these mutations into an isogenic background. Spores derived from C. difficile yabG _C207A (catalytically inactive), C. difficile yabG _A46D, C. difficile yabG _G37E, and C.

SUBMITTER: Osborne MS 

PROVIDER: S-EPMC11195116 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature