Protein-coding mutation in Adcy3 increases adiposity and alters emotional behaviors sex-dependently in rats.

ABSTRACT:

Objective

Adenylate cyclase 3 (Adcy3) has been linked to both obesity and major depressive disorder (MDD). Our lab identified a protein-coding variant in the 2^nd transmembrane (TM) helix of Adcy3 in rats, and similar obesity variants have been identified in humans. The current study investigates the role of a TM variant in adiposity and behavior.

Methods

We used CRISPR-SpCas9 to mutate the TM domain of Adcy3 in WKY rats (Adcy3^mut/mut). We also created a heterozygous knockout rat in the same strain (Adcy3^+/-). Wild-type (WT), Adcy3^+/-, and Adcy3^mut/mut rats were fed a high-fat diet for 12 weeks. We measured body weight, fat mass, glucose tolerance, food intake, metabolism, emotion-like behaviors, and

SUBMITTER: Fitzpatrick M 

PROVIDER: S-EPMC11195162 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature