Single-Cell Phenotyping of Extracellular Electron Transfer via Microdroplet Encapsulation.

ABSTRACT: Electroactive organisms contribute to metal cycling, pollutant removal, and other redox-driven environmental processes. Studying this phenomenon in high-throughput is challenging since extracellular reduction cannot easily be traced back to its cell of origin within a mixed population. Here, we describe the development of a microdroplet emulsion system to enrich EET-capable organisms. We validated our system using the model electroactive organism S. oneidensis and describe the tooling of a benchtop microfluidic system for oxygen-limited processes. We demonstrated enrichment of EET-capable phenotypes from a mixed wild-type and EET-knockout population. As a proof-of-concept application, bacteria were collected from iron sedimentation from Town Lake (Austin, TX) and subjected to microd

SUBMITTER: Partipilo G 

PROVIDER: S-EPMC11195189 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature