Widespread genomic de novo DNA methylation occurs following CD8⁺ T cell activation and proliferation.

ABSTRACT: This research investigates the intricate dynamics of DNA methylation in the hours following CD8+ T cell activation, during a critical yet understudied temporal window. DNA methylation is an epigenetic modification central to regulation of gene expression and directing immune responses. Our investigation spanned 96-h post-activation and unveils a nuanced tapestry of global and site-specific methylation changes. We identified 15,626 significant differentially methylated CpGs spread across the genome, with the most significant changes occurring within the genes ADAM10, ICA1, and LAPTM5. While many changes had modest effect sizes, approximately 120 CpGs exhibited a log₂FC above 1.5, with cell activation and proliferation pathways the most affected. Relatively fe

SUBMITTER: Seddon AR 

PROVIDER: S-EPMC11195465 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature