Probing chromatin condensation dynamics in live cells using interferometric scattering correlation spectroscopy.

ABSTRACT: Chromatin organization and dynamics play important roles in governing the regulation of nuclear processes of biological cells. However, due to the constant diffusive motion of chromatin, examining chromatin nanostructures in living cells has been challenging. In this study, we introduce interferometric scattering correlation spectroscopy (iSCORS) to spatially map nanoscopic chromatin configurations within unlabeled live cell nuclei. This label-free technique captures time-varying linear scattering signals generated by the motion of native chromatin on a millisecond timescale, allowing us to deduce chromatin condensation states. Using iSCORS imaging, we quantitatively examine chromatin dynamics over extended periods, revealing spontaneous fluctuations in chromatin condensation and heterogen

SUBMITTER: Hsiao YT 

PROVIDER: S-EPMC11196589 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature