RNAi-mediated downregulation of AcCENH3 can induce in vivo haploids in onion (Allium cepa L.).

ABSTRACT: Haploid induction (HI) holds great promise in expediting the breeding process in onion, a biennial cross-pollinated crop. We used the CENH3-based genome elimination technique in producing a HI line in onion. Here, we downregulated AcCENH3 using the RNAi approach without complementation in five independent lines. Out of five events, only three could produce seeds upon selfing. The progenies showed poor seed set and segregation distortion, and we were unable to recover homozygous knockdown lines. The knockdown lines showed a decrease in accumulation of AcCENH3 transcript and protein in leaf tissue. The decrease in protein content in transgenic plants was correlated with poor seed set. When the heterozygous knockdown lines were crossed with wild-type plants, progenies showed HI by genome elim

SUBMITTER: Manape TK 

PROVIDER: S-EPMC11196721 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature