Proof of concept of a new plasma complement Factor H from waste plasma fraction.

ABSTRACT:

Introduction

Complement factor H (FH) is a major regulator of the complement alternative pathway, its mutations predispose to an uncontrolled activation in the kidney and on blood cells and to secondary C3 deficiency. Plasma exchange has been used to correct for FH deficiency and although the therapeutic potential of purified FH has been suggested by in vivo experiments in animal models, a clinical approved FH concentrate is not yet available. We aimed to develop a purification process of FH from a waste fraction rather than whole plasma allowing a more efficient and ethical use of blood and plasma donations.

Methods

Waste fractions from industrial plasma fractionation (pooled human plasma) were analyzed for FH content by ELISA. FH was purified from unused fraction II

SUBMITTER: Mori F 

PROVIDER: S-EPMC11197005 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature