Comparison of serum and plasma SDMA measured with point-of-care and reference laboratory analysers: implications for interpretation of SDMA in cats.

ABSTRACT:

Objectives

Symmetric dimethylarginine (SDMA) reflects the glomerular filtration rate (GFR) in people, dogs and cats. Initial assays used a liquid chromatography-mass spectroscopy (LC-MS) technique. A veterinary immunoassay has been developed for use in commercial laboratories and point-of-care (POC) laboratory equipment. This study sought to: determine POC and commercial laboratory (CL) SDMA assay imprecision; determine any bias of the POC assay compared with the CL assay; calculate observed total error of the POC assay and compare with analytical performance goals; and calculate dispersion and sigma metrics (σ) for POC and CL SDMA methods.

Methods

Two separate studies were performed that assessed: (1) imprecision, determined by evaluation of pooled feline plasma or serum; a

SUBMITTER: Baral RM 

PROVIDER: S-EPMC11197123 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature