Obesity-induced Ly6C^High and Ly6C^Low monocyte subset changes abolish post-ischemic limb conditioning benefits in stroke recovery.

ABSTRACT: Remote limb conditioning (RLC), performed by intermittent interruption of blood flow to a limb, triggers endogenous tolerance mechanisms and improves stroke outcomes. The underlying mechanism for the protective effect involves a shift of circulating monocytes to a Ly6C^High proinflammatory subset in normal metabolic conditions. The current study investigates the effect of RLC on stroke outcomes in subjects with obesity, a vascular comorbidity. Compared to lean mice, obese stroke mice displayed significantly higher circulating monocytes (monocytosis), increased CD45^High monocytes/macrophages infiltration to the injured brain, worse acute outcomes, and delayed recovery. Unlike lean mice, obese mice with RLC at 2 hours post-stroke failed to shift circulating monocytes to

SUBMITTER: Kim ID 

PROVIDER: S-EPMC11197146 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature