'Small volume-big problem': culturing Yarrowia lipolytica in high-throughput micro-formats.

ABSTRACT: With the current progress in the 'design' and 'build' stages of the 'design-build-test-learn' cycle, many synthetic biology projects become 'test-limited'. Advances in the parallelization of microbes cultivations are of great aid, however, for many species down-scaling leaves a metabolic footprint. Yarrowia lipolytica is one such demanding yeast species, for which scaling-down inevitably leads to perturbations in phenotype development. Strictly aerobic metabolism, propensity for filamentation and adhesion to hydrophobic surfaces, spontaneous flocculation, and high acidification of media are just several characteristics that make the transfer of the micro-scale protocols developed for the other microbial species very challenging in this case. It is well recognized that without additional 'p

SUBMITTER: Celinska E 

PROVIDER: S-EPMC11197222 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature